SuperScript® II Reverse Transcriptase (RT) is an improved version of SuperScript® RT. It is a DNA polymerase that synthesizes a complementary DNA strand from single-stranded RNA, DNA, or an RNA:DNA hybrid. This enzyme is genetically engineered by the introduction of point mutations in the RNase H active center to reduce RNase H activity. This structural modification eliminates degradation of RNA molecules during first-strand cDNA synthesis and gives SuperScript® II RT superior performance characteristics.

• Yield – Reduced RNase H activity results in greater first-strand cDNA yields
• Size of cDNA – Generation of RT-PCR products up to 12 kb
• Thermostability – Full activity at 42°C
• Flexibility – Uses either total or poly(A)+ RNA

Performance and Quality Testing: SDS-PAGE purity; endodeoxyribonuclease, exodeoxyribonuclease, and ribonuclease assays; and yield and length of cDNA product.

Unit Definition: One unit of SuperScript® II is the amount更看更多