AF-100-18BPeprotech Recombinant Human FGF-basicpe重组人FGF-basicpe细胞生长因子的详细资料：

Recombinant Human FGF-basic （154 a.a.）

Catalog Number:

AF-100-18B

Synonyms:

Fibroblast Growth Factor-basic, FGF-2, HBGF-2, Prostatropin

Description:

FGF-basic is one of 23 known members of the FGF family. Proteins of this family play a central role during prenatal development and postnatal growth and regeneration of a variety of tissues, by promoting cellular proliferation and differentiation. FGF-basic is a non-glycosylated heparin binding growth factor that is expressed in the brain, pituitary, kidney, retina, bone, testis, adrenal gland liver, monocytes, epithelial cells and endothelial cells. FGF-basic signals through FGFR 1b, 1c, 2c, 3c and 4. Recombinant human FGF-basic is a 17.2 kDa protein consisting of 154 amino acid residues.Manufactured using all non-animal reagents.

AA Sequence:

AAGSITTLPA LPEDGGSGAF PPGHFKDPKR LYCKNGGFFL RIHPDGRVDG VREKSDPHIK LQLQAEERGV VSIKGVCANR YLAMKEDGRL LASKCVTDEC FFFERLESNN YNTYRSRKYT SWYVALKRTG QYKLGSKTGP GQKAILFLPM SAKS

Source:

E.coli

Purity:

Greater than 95% by SDS-PAGE gel and HPLC analyses.

Biological Activity:

Assay #1: The ED₅₀ as determined by the dose-dependent stimulation of thymidine uptake by BaF3 cells expressing FGF receptors is ≤ 0.5ng/ml corresponding to a specific activity of ≥ 2 x 10⁶units/mg.

Assay #2: The ED₅₀ was determined by a cell proliferation assay using balb/c 3T3 cells is ≤ 0.1 ng/ml, corresponding to a specific activity of ≥ 1 x 10⁷ units/mg.

Cross Reactivity:

Cow, Dog, Frog, Hamster, Monkey, Mouse, Pig, Rabbit, Rat, Sheep, Human + Mouse, Rat + Chicken, Human, Chicken, Horse, N/A

Research Interests: