产品目录
产品展示
当前位置:首页 > 产品中心 > 常规生化试剂类产品 > Dako抗稀释液 > Dako s3022 Dako代理进口DAKO一抗稀释液Antibody Diluent, Background Reducing

产品名称:进口DAKO一抗稀释液Antibody Diluent, Background Reducing

产品型号:Dako s3022 Dako代理

产品报价:

产品特点:for dilution of antibodies which tend to give high, non-specific background staining in immunohistochemical procedures.Diminishes background staining while maintaining adequate,specific staining

Dako s3022 Dako代理进口DAKO一抗稀释液Antibody Diluent, Background Reducing的详细资料:

Intended use For In Vitro diagnostic use.
This product is intended for use as a diluent in the preparation of primary antibodies and negative control
reagents. It is to be used with antibodies and/or antigens that yield high background in immunohistochemical
(IHC) staining procedures.
Refer to the “General Instructions for Immunohistochemical Staining” or the Detection System “Instructions” of
IHC procedures for: (1) Principle of Procedure, (2) Materials Required, Not Supplied, (3) Storage, (4) Specimen
Preparation, (5) Staining Procedure, (6) Quality Control, (7) Troubleshooting, (8) Interpretation of Staining,
(9) General Limitations.
Reagent provided 0.05 mol/L Tris-HCl buffer containing 0.1% Tween, stabilizing proteins to reduce background, and 0.015 mol/L
sodium azide.
Precautions 1. For professional users.
2. This product contains sodium azide (NaN3), a chemical highly toxic in pure form. At product concentrations,
though not classified as hazardous, build-ups of NaN3 may react with lead and copper plumbing to form
highly explosive metal azides. Upon disposal, flush with large volumes of water to prevent azide build-up in
plumbing.1,2
3. Wear appropriate Personal Protective Equipment to avoid contact with eyes and skin.
4. Unused solution should be disposed of according to local, State and Federal regulations.
Reactivity For demonstration of tissue antigens by IHC, optimal staining performance is dependent on the correct dilution
of the primary antibody. The interaction between antibodies and epitopes consists mainly of relatively weak
forces such as van der Waals forces, electrostatic forces and hydrophobic forces, which are influenced by pH
and ionic strength. Consequently, to assure optimal performance of the antibody with the least amount of
background, the choice of diluent is important.
Applications This product provides an appropriay buffered solution for the dilution of both polyclonal and monoclonal
antibodies and for the preparation of negative control reagents in IHC. This product is particularly useful for
reducing background staining while maintaining specific reactivity. Antibody Diluent with Background Reducing
Components can also be used to stabilize diluted antibodies when stored at 2–8°C. This diluent should be
brought to room temperature and mixed thoroughly prior to use.
Limitations Tissue staining results are dependent upon the handling and processing of the tissue prior to staining.
Aldehyde-containing fixatives, such as formalin and glutaraldehyde frequently contribute to increased
background staining. Improper fixation, freezing, thawing, washing, drying, heating or sectioning may produce
artifacts, antibody trapping or false-negative results. Also, false-positive staining may be caused by crossreactivity
of other IHC staining reagents to e.g. endogenous alkaline phosphatase, endogenous peroxidase,
pseudoperoxidase, endogenous avidin-binding activity and/or by nonspecific reaction with necrotic or
degenerated cells.
Storage Store at 2–8°C. Do not freeze.

 

 

上海力敏实业有限公司代理Dako产品,咨询 
上海力敏实业有限公司代理Dako产品,咨询 

 如果你对Dako s3022 Dako代理进口DAKO一抗稀释液Antibody Diluent, Background Reducing感兴趣,想了解更详细的产品信息,填写下表直接与厂家联系:

留言框

  • 产品:

  • 留言内容:

  • 您的单位:

  • 您的姓名:

  • 联系电话:

  • 常用邮箱:

  • 详细地址:

  • 省份:

  • 验证码:

    请输入计算结果(填写阿拉伯数字),如:三加四=7
上一篇:Roche产品价格优惠11243233001Roche代理 琼脂糖凝胶G蛋白填料Protein G-Agarose罗氏产品供应 下一篇:Hyclone SH30074.03 500mlHyclone供体马血清