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产品名称:Anti-Mannose Receptor antibody 5C11甘露糖受体抗体

产品型号:abcam ab117644

产品报价:

产品特点:Anti-Mannose Receptor antibody
Tested applications
WB, IHC-P, Sandwich ELISA
Cross reactivity
Reacts with
Human, Monkey

abcam ab117644Anti-Mannose Receptor antibody 5C11甘露糖受体抗体的详细资料:

Overview

Product name

Anti-Mannose Receptor antibody [5C11]
See all Mannose Receptor products (6) ...

Description

Mouse monoclonal [5C11] to Mannose Receptor

Tested applications

WB, IHC-P, Sandwich ELISAmore details

Cross reactivity

 

Reacts with

Human, Monkey

 

Immunogen

Recombinant Human Mannose Receptor protein (NP_002429.1).

Positive control

Human lung (macrophages) tissue; Human pancreas lysate.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Storage buffer

pH: 7.20
Constituent: 99% PBS

Concentration

 

  • 50 µg at 0.5mg/ml

Purity

Protein A purified

Clonality

Monoclonal

Clone number

5C11

Isotype

IgG1

Light chain type

kappa

Research areas

Immunology >> Immunoglobulins >> Receptors
Immunology >> Innate Immunity >> Macrophage / Inflamm.
 

  • Datasheet PDF
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mannose Receptor antibody [5C11] (ab117644)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mannose Receptor antibody [5C11] (ab117644) image(enlarge)

  • Western blot - Anti-Mannose Receptor antibody [5C11] (ab117644)Western blot - Anti-Mannose Receptor antibody [5C11] (ab117644) image(enlarge)

  • Sandwich ELISA - Anti-Mannose Receptor antibody [5C11] (ab117644)Sandwich ELISA - Anti-Mannose Receptor antibody [5C11] (ab117644) image(enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab117644 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ShowHide1 Image

    WB

    WB: 1/500 - 1/1000. Pred...Read more →

    WB: 1/500 - 1/1000. Predicted molecular weight: 166 kDa.

  • ShowHide2 Images

    IHC-P

    (1 AbreviewIHC-P: Use a concentrati...Read more →

    IHC-P: Use a concentration of 5 µg/ml.

  • ShowHide1 Image

    Sandwich ELISA

    sELISA: Use at an assay ...Read more →

    sELISA: Use at an assay dependent concentration.

Target

Function

Mediates the endocytosis of glycoproteins by macrophages. Binds both sulfated and non-sulfated polysaccharide chains. Acts as phagocytic receptor for bacteria, fungi and other pathogens.

Sequence similarities

Contains 8 C-type lectin domains.
Contains 1 fibronectin type-II domain.
Contains 1 ricin B-type lectin domain.

Cellular localization

Membrane.

Information by UniProt

Alternative names

  • C-type lectin domain family 13 member D antibody
  • CD 206 antibody
  • CD206 antibody
see all

Database links

  • Entrez Gene: 4360 Human
  • Omim: 153618 Human
  • SwissProt: P22897 Human
  • Unigene: 75182 Human

Anti-Mannose Receptor antibody [5C11] images:

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mannose Receptor antibody [5C11] (ab117644)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mannose Receptor antibody [5C11] (ab117644)

 

 

ab117644, at 5 µg/ml, staining Mannose Receptor in Formalin-fixed, Paraffin-embedded Human Lung (Macrophages) tissue by Immunohistochemistry.

 

Western blot - Anti-Mannose Receptor antibody [5C11] (ab117644)

Western blot - Anti-Mannose Receptor antibody [5C11] (ab117644)

 

 

Anti-Mannose Receptor antibody [5C11] (ab117644) + Human pancreas lysate

Predicted band size : 166 kDa
 

 

Sandwich ELISA - Anti-Mannose Receptor antibody [5C11] (ab117644)

Sandwich ELISA - Anti-Mannose Receptor antibody [5C11] (ab117644)

 

 

Detection limit for recombinant tagged Mannose Receptor is approximay 0.03ng/ml as a capture antibody.

 

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mannose Receptor antibody [5C11] (ab117644)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mannose Receptor antibody [5C11] (ab117644)

 

 

ab117644 staining Mannose Receptor in rhesus monkey brain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).

Tissue was fixed with formaldehyde and blocked with 20% serum for 1 hour at 37°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/200 in 10% serum in 20% Normal Horse Serum in 1X TTBS) for 12 hours at 37°C. A biotin-conjugated horse anti-mouse IgG polyclonal (1/200) was used as the secondary antibody.

This image is courtesy of an Abreview submitted by Mr Peter Sullivan

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