产品目录
产品展示
当前位置:首页 > 产品中心 > 抗体 > Abcam抗体 > abcam ab13918Anti-RANK antibody

产品名称:Anti-RANK antibody

产品型号:abcam ab13918

产品报价:

产品特点:Anti-RANK antibody
Tested applications
IHC-P, IP, WB, Flow Cyt
Cross reactivity
Reacts with
Mouse, Human

abcam ab13918Anti-RANK antibody的详细资料:

Overview

Product name

Anti-RANK antibody [64C1385]
See all RANK products (9) ...

Description

Mouse monoclonal [64C1385] to RANK

Tested applications

IHC-P, IP, WB, Flow Cytmore details

Cross reactivity

 

Reacts with

Mouse, Human

 

Immunogen

Recombinant fragment containing the extracellular domain of RANK (Human).

Positive control

Activated T cells or RAW (a mouse macrophage monocyte).

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 0.02% Sodium Azide
Constituents: PBS

Concentration

 

  • 100 µg at 0.5mg/ml

Purity

Protein G purified

Clonality

Monoclonal

Clone number

64C1385

Isotype

IgG1

Research areas

Cardiovascular >> Atherosclerosis >> Vascular Inflammation >> Inflammatory mediators
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> Structures >> Bone
Signal Transduction >> Growth Factors/Hormones >> TNF
Signal Transduction >> Adapters >> Transmembrane
Immunology >> Innate Immunity >> Cytokines >> TNF Superfamily
 

  • Datasheet PDF
  • Immunoprecipitation - RANK antibody [64C1385] (ab13918)Immunoprecipitation - RANK antibody [64C1385] (ab13918) image(enlarge)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RANK antibody [64C1385] (ab13918)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) -  [64C1385] (ab13918) image(enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab13918 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ShowHide1 Image

    IHC-P

    IHC-P: Use a concentrati...Read more →

    IHC-P: Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • ShowHide1 Image

    IP

    IP: Use a concentration ...Read more →

    IP: Use a concentration of 1 - 2 µg/ml.

  • ShowHide

    WB

    WB: Use a concentration ...Read more →

    WB: Use a concentration of 1 - 2 µg/ml. Detects a band of approximay 97 kDa.

  • ShowHide

    Flow Cyt

    See more...Read more →

    Flow Cyt: Use 5µg for 106 cells.

Target

Function

Receptor for TNFSF11/RANKL/TRANCE/OPGL; essential for RANKL-mediated osteoclastogenesis. Involved in the regulation of interactions between T-cells and dendritic cells.

Tissue specificity

Ubiquitous expression with high levels in skeletal muscle, thymus, liver, colon, small intestine and adrenal gland.

Involvement in disease

Defects in TNFRSF11A are the cause of familial expansile osteolysis (FEO) [MIM:174810]. FEO is a rare autosomal dominant bone disorder characterized by focal areas of increased bone remodeling. The osteolytic lesions develop usually in the long bones during early adulthood. FEO is often associated with early onset deafness and loss of dentition.
Defects in TNFRSF11A are a cause of Paget disease of bone type 2 (PDB2) [MIM:602080]; also known as familial Paget disease of bone. PDB2 is a bone-remodeling disorder with clinical similarities to FEO. Unlike FEO, however, affected individuals have involvement of the axial skeleton with lesions in the spine, pelvis and skull.
Defects in TNFRSF11A are the cause of osteopetrosis autosomal recessive type 7 (OPTB7) [MIM:612301]; also called osteoclast-poor osteopetrosis with hypogammaglobulinemia. Osteopetrosis is a rare genetic disease characterized by abnormally dense bone, due to defective resorption of immature bone. The disorder occurs in two forms: a severe autosomal recessive form occurring in utero, infancy, or childhood, and a benign autosomal dominant form occurring in adolescence or adulthood. OPTB7 is characterized by paucity of osteoclasts, suggesting a molecular defect in osteoclast development. OPTB7 is associated with hypogammaglobulinemia.

Sequence similarities

Contains 4 TNFR-Cys repeats.

Cellular localization

Membrane.

Information by UniProt

Alternative names

  • Activator of NFKB antibody
  • CD 265 antibody
  • CD265 antibody
see all

Database links

  • Entrez Gene: 8792 Human
  • Entrez Gene: 21934 Mouse
  • Omim: 603499 Human
  • SwissProt: Q9Y6Q6 Human
  • SwissProt: O35305 Mouse
  • Unigene: 204044 Human
  • Unigene: 6251 Mouse

[64C1385] images:

Immunoprecipitation - RANK antibody [64C1385] (ab13918)

Immunoprecipitation - RANK antibody [64C1385] (ab13918)

 

 

 

Detection of RANK in RAW cells. Lane 1: mouse cell line. Lane 2 & 3: IP/Western blot analysis of RANK. RANK protein from RAW cell lysate was immunoprecipitated either with control antibody (lane 2) or ab13918 (lane 3), and detected with ab13918.

 

 

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - [64C1385] (ab13918)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) -  [64C1385] (ab13918)

 

 

IHC image of ab13918 staining in human t cell lymphoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab13918, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

 

References for [64C1385] (ab13918)

 如果你对abcam ab13918Anti-RANK antibody感兴趣,想了解更详细的产品信息,填写下表直接与厂家联系:

留言框

  • 产品:

  • 留言内容:

  • 您的单位:

  • 您的姓名:

  • 联系电话:

  • 常用邮箱:

  • 详细地址:

  • 省份:

  • 验证码:

    请输入计算结果(填写阿拉伯数字),如:三加四=7
上一篇:RPN303BAmersham Hybond-N+ 带正电荷尼龙膜0.45um,30cmx3m 下一篇:abcam ab846Anti-CD4 antibody