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产品名称:Pronase E 蛋白酶EProtease

产品型号:sigmaP6911

产品报价:

产品特点:Protease from Streptomyces griseus
别名: Actinase E Pronase E
产品规格:100mg 1g 5g

sigmaP6911Pronase E 蛋白酶EProtease的详细资料:

产品名称

Application

Protease is typically used in nucleic acid isolation procedures in incubations of 0.5-3.0 hours supplemented with 0.2% sodium dodecyl sulfate and 10 mM EDTA.

 

Protease is used to degrade and remove protein during nucleic acid purification and other molecular biological methods.

Preparation Note

Collected from culture broth of S. griseus and purified by successive column procedures.

Specificity

A mixture of at least three proteolytic activities including an extracellular serine protease. In general, serine proteases display a wide range of substrate specificities, which are believed to be mediated by an active site composed of one Asp, one His, and a Ser residue in the molecule. This enzyme prefers to hydrolyze peptide bonds on the carboxyl side of glutamic or aspartic acid.

Unit Definition

One unit will hydrolyze casein to produce color equivalent to 1.0 μmole (181 μg) of tyrosine per min at pH 7.5 at 37 °C (color by Folin-Ciocalteu reagent).

Physical properties

Compley inactivated by heating above 80 °C for 15-20 minutes.

价格与基本信息

Related Categories Application Index, Biochemicals and Reagents, Enzymes, Inhibitors, and Substrates, Modifying Enzymes, Molecular Biology,
更多...
grade for molecular biology
product line BioReagent
activity ≥4 units/mg solid
mol wt monomer mol wt ~20 kDa
foreign activity DNase, RNase, and nickase, none detected (No RNase.)
storage temp. −20°C

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