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产品名称:Anti-Flt3 ligand antibody

产品型号:ab52648

产品报价:

产品特点:Tested applications
ICC/IF, WB, Flow Cyt, IHC-P
Cross reactivity
Reacts with
Human

ab52648Anti-Flt3 ligand antibody的详细资料:

Overview

Product name

Anti-Flt3 ligand antibody [EP1140Y]
See all Flt3 ligand products (6) ...

Description

Rabbit monoclonal [EP1140Y] to Flt3 ligand

Specificity

The antibody has been tested against recombinant protein in western blotting, we do not have data regarding recognition of endogenous protein. We suggest K562 and HL-60 cells could be expressing Flt3 ligand. The antibody was raised against the extracellular domain of the protein, however please note permeabilisation in FACS greatly improves the signal.

Tested applications

ICC/IF, WB, Flow Cyt, IHC-Pmore details

Cross reactivity

 

Reacts with

Human

Does not react with

Mouse, Rat

 

Immunogen

A synthetic peptide corresponding to residues within human Flt3 ligand.

Positive control

IHC: Human tonsil tissue. WB: recombinant protein

General notes

Produced under U.S. Patent No. 5,675,063.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Storage buffer

PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%

Purity

Tissue culture supernatant

Clonality

Monoclonal

Clone number

EP1140Y

Isotype

IgG

Research areas

Immunology >> Innate Immunity >> Cytokines >> Other
Cardiovascular >> Angiogenesis >> Cytokines >> Other
 

  • Datasheet PDF
  • Western blot - Flt3 ligand antibody [EP1140Y] (ab52648)Western blot - Flt3 ligand antibody [EP1140Y] (ab52648) image(enlarge)

  • Immunohistochemistry (Paraffin-embedded sections) - Flt3 ligand antibody [EP1140Y] (ab52648)Immunohistochemistry (Paraffin-embedded sections) - Flt3 ligand antibody [EP1140Y] (ab52648) image(enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab52648 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ICC/IF

     
  • 1 Image

    WB

     
  • Flow Cyt

     
  • 1 Image

    IHC-P

     

Application notes

Flow Cyt: 1/10.
ICC/IF: 1/100 - 1/250.
IHC-P: 1/50 - 1/100. Antigen retrieval recommended.
WB: 1/100000. Detects a band of approximay 22 kDa (predicted molecular weight: 26 kDa).

Is unsuitable for IP.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

Function

Stimulates the proliferation of early hematopoietic cells by activating FLT3. Synergizes well with a number of other colony stimulating factors and interleukins.

Cellular localization

Secreted and Cell membrane.

Information by UniProt

Alternative names

  • Flt 3 ligand antibody
  • Flt 3L antibody
  • Flt3 L antibody
see all

Database links

  • Entrez Gene: 2323 Human
  • Omim: 600007 Human
  • SwissProt: P49771 Human
  • Unigene: 428 Human

Anti-Flt3 ligand antibody [EP1140Y] images:

Western blot - Flt3 ligand antibody [EP1140Y] (ab52648)

Western blot - Flt3 ligand antibody [EP1140Y] (ab52648)

 

 

[EP1140Y] (ab52648) at 1/100000 dilution + Recombinant Flr3 ligand at 10 µg

Secondary
Goat anti-rabbit HRP labeled at 1/2000 dilution

Predicted band size : 26 kDa
Observed band size : 22 kDa (why is the actual band size different from the predicted?)
 

 

Immunohistochemistry (Paraffin-embedded sections) - Flt3 ligand antibody [EP1140Y] (ab52648)

Immunohistochemistry (Paraffin-embedded sections) - Flt3 ligand antibody [EP1140Y] (ab52648)

 

 

Immunohistochemical staining of paraffin-embedded human tonsils using ab52648 at a 1/50 dilution.

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