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产品名称:Anti-Osteoprotegerin antibody反骨保护素抗体

产品型号:abcam ab73400

产品报价:

产品特点:ICC/IF, IHC-P, WB
Reacts with
Human
Predicted to work with
Mouse, Rat, Sheep, Cow, Pig

abcam ab73400Anti-Osteoprotegerin antibody反骨保护素抗体的详细资料:

Overview

Product name

Anti-Osteoprotegerin antibody
See all Osteoprotegerin products (16) ...

Description

Rabbit polyclonal to Osteoprotegerin

Tested applications

ICC/IF, IHC-P, WBmore details

Cross reactivity

 

Reacts with

Human

Predicted to work with

Mouse, Rat, Sheep, Cow, Pig

 

Immunogen

Synthetic peptide conjugated to KLH derived from within residues 200 - 300 Osteoprotegerin.

Read Abcam's proprietary immunogen policy

 

(Peptide available as ab733 99.)

Positive control

This antibody gave a positive signal in the following human whole cell lysates: SK N BE and K562

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4

Concentration

 

  • 100 µg at 1mg/ml

Purity

Immunogen affinity purified

Clonality

Polyclonal

Isotype

IgG

Research areas

Metabolism >> Types of disease >> Obesity
Cardiovascular >> Atherosclerosis >> Vascular Inflammation >> Inflammatory mediators
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> Structures >> Bone
Immunology >> Innate Immunity >> Cytokines >> TNF Superfamily
Cardiovascular >> Blood >> Other
 

  • Datasheet PDF
  • Western blot - Anti-Osteoprotegerin antibody (ab73400)Western blot - Anti-Osteoprotegerin antibody (ab73400) image(enlarge)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Osteoprotegerin antibody (ab73400)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Osteoprotegerin antibody (ab73400) image(enlarge)

  • Immunocytochemistry/ Immunofluorescence - Osteoprotegerin antibody (ab73400)Immunocytochemistry/ Immunofluorescence - Osteoprotegerin antibody (ab73400) image(enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab73400 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ShowHide1 Image

    ICC/IF

    ICC/IF: Use a concentrat...Read more →

    ICC/IF: Use a concentration of 5 µg/ml.

  • ShowHide1 Image

    IHC-P

    IHC-P: Use a concentrati...Read more →

    IHC-P: Use a concentration of 5 µg/ml.

  • ShowHide1 Image

    WB

    WB: Use a concentration ...Read more →

    WB: Use a concentration of 1 µg/ml. Detects a band of approximay 48 kDa (predicted molecular weight: 46 kDa).

Target

Function

Acts as decoy receptor for RANKL and thereby neutralizes its function in osteoclastogenesis. Inhibits the activation of osteoclasts and promotes osteoclast apoptosis in vitro. Bone homeostasis seems to depend on the local RANKL/OPG ratio. May also play a role in preventing arterial calcification. May act as decoy receptor for TRAIL and protect against apoptosis. TRAIL binding blocks the inhibition of osteoclastogenesis.

Tissue specificity

Highly expressed in adult lung, heart, kidney, liver, spleen, thymus, prostate, ovary, small intestine, thyroid, lymph node, trachea, adrenal gland, testis, and bone marrow. Detected at very low levels in brain, placenta and skeletal muscle. Highly expressed in fetal kidney, liver and lung.

Involvement in disease

Defects in TNFRSF11B are the cause of juvenile Paget disease (JPD) [MIM:239000]; also known as hyperostosis corticalis deformans juvenilis or hereditary hyperphosphatasia or chronic congenital idiopathic hyperphosphatasia. JPD is a rare autosomal recessive osteopathy that presents in infancy or early childhood. The disorder is characterized by rapidly remodeling woven bone, osteopenia, debilitating fractures, and deformities due to a markedly accelerated rate of bone remodeling throughout the skeleton. Approximay 40 cases of JPD have been reported worldwide. Unless it is treated with drugs that block osteoclast-mediated skeletal resorption, the disease can be fatal.

Sequence similarities

Contains 2 death domains.
Contains 4 TNFR-Cys repeats.

Post-translational
modifications

N-glycosylated. Contains sialic acid residues.
The N-terminus is blocked.

Cellular localization

Secreted.

Information by UniProt

Alternative names

  • MGC29565 antibody
  • OCIF antibody
  • OPG antibody
see all

Database links

  • Entrez Gene: 4982 Human
  • Entrez Gene: 18383 Mouse
  • Entrez Gene: 25341 Rat
  • Omim: 602643 Human
  • SwissProt: O00300 Human
  • SwissProt: O08712 Mouse
  • SwissProt: O08727 Rat
  • Unigene: 81791 Human
see all

Anti-Osteoprotegerin antibody images:

Western blot - Anti-Osteoprotegerin antibody (ab73400)

Western blot - Anti-Osteoprotegerin antibody (ab73400)

 

 

All lanes : Anti-Osteoprotegerin antibody (ab73400) at 1 µg/ml

Lane 1 : SK N BE (Human neuroblastoma) Whole Cell Lysate
Lane 2 : K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate

Lysates/proteins at 10 µg per lane.

Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Performed under reducing conditions.

Predicted band size : 46 kDa
Observed band size : 48 kDa (why is the actual band size different from the predicted?)
Additional bands at : 105 kDa,14 kDa. We are unsure as to the identity of these extra bands.

Exposure time : 8 minutes

Osteoprotegerin contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.

 

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Osteoprotegerin antibody (ab73400)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Osteoprotegerin antibody (ab73400)

 

 

IHC image of Osteoprotegerin staining in Human Normal Kidney FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab73400, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

 

Immunocytochemistry/ Immunofluorescence - Osteoprotegerin antibody (ab73400)

Immunocytochemistry/ Immunofluorescence - Osteoprotegerin antibody (ab73400)

 

 

ICC/IF image of ab73400 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab73400, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5 min) HeLa, Hek293 and HepG2 cells at 5µg/ml.

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