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今日推荐THERMO T4 DNA连接酶EL0014
点击次数:1200 发布时间:2017-08-29

今日推荐THERMO T4 DNA连接酶EL0014

Thermo Scientific T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA. The enzyme repairs single-strand nicks in duplex DNA, RNA, or DNA/RNA hybrids. It also joins DNA fragments with either cohesive or blunt termini, but has no activity on single-stranded nucleic acids.

T4 DNA Ligase requires ATP as a cofactor.

Highlights

• Active in Themo Scientific restriction enzyme, PCR, and RT buffers (when supplemented with ATP)
• Fast—sticky-end ligation is completed in 10 minutes at room temperature
• Supplied with PEG solution for efficient blunt-end ligation

THERMO T4 DNA连接酶,T4 DNA Ligase

订购信息:

 

货号

单位 
 EL0011

1,000 units

  
 EL0012

5 x 1,000 units

  
 EL0014

200 units

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